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2.5 RNA isolation and quantitative real-time PCR Total RNA was extracted from HCECs using the TRIzol reagent (Vazyme Biotech Co., Ltd., Nanjing, China), according to the manufacturers instructions

After 48 h, these mice were treated with a daily intraperitoneal injection of JMF4073 for 14 days (Fig

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Oxidative stress and mitochondrial impairment can be separated from lipofuscin accumulation in aged human skeletal muscle

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